Fig 2: Natural GSNOR variants cause variation of root growth tolerance to high Fe. a Schematic of the constructs for GSNOR variants to generate transgenic lines. b Box plots for root length of T2 seedlings of ten GSNOR_Sf-2 lines and four GSNOR_Col-0 lines at day 6. hot5-4 and Ws-4 were included as the comparison for high Fe sensitivity. Not detected (nd) indicates no root growth of hot5-4 was observed at 350 µM Fe conditions. c Box plots for the mean of ten GSNOR_Sf-2 lines and four GSNOR_Col-0 lines shown in b. Numbers below and next to boxes in b, c indicate the number (n) of T2 seedlings used for each line in the analysis. Different letters indicate significant difference according to Tukey’s HSD comparison (p-value < 0.05). The Source data of Fig. 4b are provided in a Source Data file

Fig 3: GSNOR is required for root tolerance to high Fe. a Seedlings of gsnor knockout mutants (hot5-2 and hot5-4) and the respective wild-type (Col-0 and Ws-4) upon high Fe (250 µM) treatment for 6 days. Scale bar, 5 mm. b Mean root length of wild-type (Col-0), gsnor mutant (hot5-2), complemented line (GSNOR-GFP), and over-expression (GSNOR-OE) line. Error bars: standard deviation. n = 15 biologically independent samples. Asterisk * and ** represent the significant differences between high Fe (150 µM Fe in blue asterisk, 250 µM Fe in red asterisk) and the control as determined by Student’s t-test at p-value < 0.05 and 0.01, respectively. The source data of Fig. 3b are provided in a Source Data file